Preliminary tests have indicated that DNA found on the body of Hannah Witheridge does not match DNA from David Miller, Thai police have confirmed.
Officers have told reporters that the samples also do not match those from brothers Christopher and James Ware, who were childhood friends of Mr Miller questioned as part of the investigation.
Some 11 Burmese migrants on the island of Koh Tao had been arrested after bloodstains were found on some of their clothes by police.
But investigators are under renewed pressure after DNA tests on the group also failed to bring any matches.
David Miller died from drowning and a blow to the head, police have claimedAdditionally, the results indicated that semen discovered on Miss Witheridge's semi-naked body does not belong to David Miller.
This could undermine a previous theory that the two victims had been having sex on a beach shortly before their deaths, considered a taboo in the deeply religious country.
Chris Ware, and his brother Jonathan, have been asked to stay in ThailandOn Wednesday night, police had confirmed there was forensic evidence to suggest that the 23-year-old woman, from Great Yarmouth, might have been raped.
Meanwhile, the Prime Minister of Thailand, General Prayuth Chan-ocha, has caused controversy by apparently suggesting that good-looking women put themselves in danger by assuming his country is safe.
He was quoted as saying: "Can they be safe in bikinis... unless they are not beautiful?"
The military ruler later issued an apology for causing alarm amongst tourists. It read: "I am sorry that my statement caused uneasiness. I affirm that I did not look down on or criticise anyone. I simply wanted to warn them to be careful at certain places and certain times."
Hannah Witheridge's family hug each other as they prepare to meet policeOfficers have said that full DNA results will be released later today. The family of one of the victims arrived in Thailand early on Thursday.
Miss Witheridge and Mr Miller were found in the early hours of Monday morning. Both had sustained head wounds, and a bloodstained garden hoe - believed to be the murder weapon - was found nearby.
It has been revealed that Mr Miller, a 24-year-old from Jersey, died from drowning in conjunction with a blow to the head, while wounds on his hands indicate a struggle had taken place.
Both of their families have paid emotional tributes, with Miss Witheridge's relatives describing her as "a beautiful, intelligent, loving young woman who poured joy into the lives of all who knew her".
Mr Miller was described as "hardworking, bright and conscientious, with everything to look forward to".
Police are looking for a man seen running past a CCTV camera near the beachJames and Christopher Ware have not been detained by police, nor have they been declared as suspects in the killings. They had been asked to remain on the island, but may be allowed to fly home shortly.
Police say they are keen to trace an Asian man captured on grainy CCTV footage near the scene of the murders.
He was seen running past a security camera at 3.44am on Monday morning, and walked down the same street an hour later.
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